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Individualized Pixel Synthesis and Characterization of Combinatorial Materials Chips Article

Xiao-Dong Xiang, Gang Wang, Xiaokun Zhang, Yong Xiang, Hong Wang

Engineering 2015, Volume 1, Issue 2,   Pages 225-233 doi: 10.15302/J-ENG-2015041

Abstract: Combinatorial materials chip technology, featuring high-throughput synthesis and characterization, ispulse of energy to heat at different temperatures while monitoring the phase evolution on the pixel <em>in situem> in real time.

Keywords： combinatorial materials chip     phase diagram     pixel synthesis     <em>in-situem> characterization     phase-boundary    

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Direct Evidence of Coal Swelling and Shrinkage with Injecting CO2 and N2 Using <em> in-situem> Synchrotron X-ray Microtomography Article

Guanglei Zhang, P.G. Ranjith, Herbert E. Huppert

Engineering 2022, Volume 18, Issue 11,   Pages 88-95 doi: 10.1016/j.eng.2022.03.010

Abstract: Here, using in situ synchrotron X-ray microtomography, we provide the first observational evidence that

Keywords： CCS     CO2     -ECBM     Carbon neutrality     X-ray imaging     Coal permeability    

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The Use of Data Mining Techniques in Rockburst Risk Assessment

Luis Ribeiro e Sousa, Tiago Miranda, Rita Leal e Sousa, Joaquim Tinoco

Engineering 2017, Volume 3, Issue 4,   Pages 552-558 doi: 10.1016/J.ENG.2017.04.002

Abstract: In this paper, we focus on the analysis of a database of <em>in situem> cases of rockburst in orderThe <em>in situem> rockburst database was further analyzed using different DM techniques ranging from

Keywords： Rockburst     Data mining     Bayesian networks     <em>In situem> database    

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Serum <em>Nem>-Glycan Markers for Diagnosing Significant Liver Fibrosis and Cirrhosis in Chronic Hepatitis Article

Lin Wang, Yiqi Liu, Qixin Gu, Chi Zhang, Lei Xu, Lei Wang, Cuiying Chen, Xueen Liu, Hong Zhao, Hui Zhuang

Engineering 2023, Volume 26, Issue 7,   Pages 151-158 doi: 10.1016/j.eng.2023.03.008

Abstract:

The aim of this study was to explore the role of serum <em>Nem>-glycomic-derived models in diagnosingSerum <em>Nem>-glycan profiles were tested using DNA sequencer-assisted fluorophore-assisted carbohydrateA total of nine <em>Nem>-glycan peaks were identified in serum samples for each subject.In distinguishing significant liver fibrosis, the diagnostic efficiency of the serum <em>Nem>-glycanIn distinguishing liver cirrhosis, the diagnostic AUROC curve of the serum <em>Nem>-glycan RF model

Keywords： Liver fibrosis     Chronic hepatitis B     Serum <em>Nem>-glycan     <em>Nem>-glycan model     Alanine aminotransferase    

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Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

Frontiers of Medicine 2023, Volume 17, Issue 2,   Pages 240-262 doi: 10.1007/s11684-022-0936-z

Abstract: After identifying conserved gene modules co-expressed with EGFR or PDGFRA (EM or PM), we recently proposedan EM/PM classification scheme for adult gliomas in a histological subtype- and grade-independent mannerRNA-seq samples, and clinical samples, we here demonstrate the temporal and spatial stability of the EMThe EM and PM subtypes, which progress in a subtype-specific mode, are robustly maintained in pairedWithin individual gliomas, the EM/PM subtype was preserved across regions and single cells.

Keywords： glioma progression     molecular classification     EM/PM subtyping     intratumor heterogeneity    

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Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 6, doi: 10.1007/s11783-016-0884-4

Abstract: Flow cytometry based on nucleic acid stains and CTC was established and optimized. Membrane of is more resistant to chlorine/chloramine than . The metabolic activity of bacteria persisted after the cytomembranewas damaged. showed more resistance to UV irradiation than by FCM. MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Flow cytometry (FCM) has been widely used in multi-parametric assessment of cells in various research fields, especially in environmental sciences. This study detected the metabolic activity of and by using an FCM method based on 5-cyano-2,3-ditolyltetrazolium chloride (CTC); the accuracy of this method was enhanced by adding SYTO 9 and 10% R2A broth. The disinfection effects of chlorine, chloramine, and UV were subsequently evaluated by FCM methods. Chlorine demonstrated stronger and faster destructive effects on cytomembrane than chloramine, and nucleic acids decomposed afterwards. The metabolic activity of the bacteria persisted after the cytomembranewas damaged as detected using CTC. Low-pressure (LP) UV or medium-pressure (MP) UV treatments exerted no significant effects on membrane permeability. The metabolic activity of the bacteria decreased with increasing UV dosage, and MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Furthermore, the membrane of Gram-positive was more resistant to chlorine/chloramine than that of Gram-negative . In addition, showed higher resistance to UV irradiation than .

Keywords： Flow cytometry     <em>Escherichia coliem>     <em>Staphylococcusaureusem>     UV     CTC     SYTO 9    

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Comprehensive kinetostatic modeling and morphology characterization of cable-driven continuum robots

Frontiers of Mechanical Engineering 2023, Volume 18, Issue 3, doi: 10.1007/s11465-023-0756-0

Abstract: In-situ maintenance is of great significance for improving the efficiency and ensuring the safetyemerged as a novel tool that aims to promote the development of intelligence and efficiency for in-situkinematic and morphology of CDCR lays the foundation for the accurate operation and control for in-situsystem is integrated to experimentally validate the proposed kinetostatic model and the concept of in-situThe compact continuum robot system could be considered a novel solution to perform in-situ maintenance

Keywords： kinetostatic modeling     morphology characterization     variable friction     continuum robots     in-situ   

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Profound Diversity of the <em>Nem>-Glycome from Microdissected Regions of Colorectal Cancer, Stroma Article

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Engineering 2023, Volume 26, Issue 7,   Pages 32-43 doi: 10.1016/j.eng.2022.08.016

Abstract: While the <em>Nem>-glycosylation of cell lines and serum has been widely examined, the analysis ofcancer-associated <em>Nem>-glycans from tissues has been hampered by the heterogeneity of tumors andthe complexity of <em>Nem>-glycan structures.ionization tandem mass spectrometry, enabling the differentiation and structural characterization of>Nem>-glycans, as well as a decrease in bisected <em>Nem>-glycans.

Keywords： Colorectal cancer     Tumor     Porous graphitized carbon liquid chromatography mass spectrometry     N-glycomics     Antibody response    

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Conversion Mechanism of Biomass to Nano Zero-Valent Iron Biochar: Iron Phase Transfer and In situ Reduction Article

Shengnan Zhuo, Hongyu Ren, Guojun Xie, Defeng Xing, Bingfeng Liu

Engineering 2023, Volume 21, Issue 2,   Pages 124-134 doi: 10.1016/j.eng.2021.07.012

Abstract:

Nano zero-valent iron biochar (nZVI–BC), an environmentally-friendly functional material prepared from waste biomass, has attracted extensive attention. This material has potential to solve the problem of biomass conversion. However, the lack of a method of converting biomass to the nZVI–BC involved in biomass modification and pyrolysis hinders its further production and application. In this study, we introduced the green solvent polyethylene glycol 400 (PEG400) to a biomass (rice straw, RS) modification system with FeCl3·6H2O, and activated RS was prepared to nZVI–BC by one-step pyrolysis. The addition of PEG400 promoted the hydrolysis of iron ion and improved the RS surface structure, promoting the attachment of Fe2O3 to the RS surface. The mild activation conditions with temperatures of 60, 80, 100 °C and a time of 0.5 h prevented the excessive loss of the lignin component and were conducive to the formation of carbon skeletons. Amorphous carbon and Fe2O3 were subjected to redox reactions to form nZVI–BC with the assistance of reducing gas produced from pyrolysis. In addition, the prepared nZVI–BC was tested for dye (Congo red) removal, showing rapid absorption (70.6% at 5 min) and high catalysis in advanced oxidation (75.67% at 5 min, 90% at 60 min). This work proposed a novel mechanistic strategy for preparing nZVI–BC and set a foundation for its scaled production and application.

Keywords： Conversion mechanism     Biomass modification     nZVI-biochar preparation     <em>In situem> pyrolysis     Iron    

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Polyolefin-nanocomposites with special properties by in-situ polymerization

Walter Kaminsky

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 3,   Pages 555-563 doi: 10.1007/s11705-018-1715-x

Abstract:

Exceptionally strong polyolefin nanocomposites are synthesized by in-situ polymerization using

Keywords： polyolefin nanocomposites     metallocenes     methylaluminoxane    

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VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 4,   Pages 542-551 doi: 10.1007/s11705-016-1599-6

Abstract: We present the microbial green synthesis of silver nanoparticles (NPs) by VITHM1 strain (MTCC No. 12465). The secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO , mediated the biological synthesis of AgNPs. The synthesized AgNPs were characterized by UV-visible spectrum, X-ray diffraction (XRD), atomic force microscope, scanning electron microscopy equipped with energy dispersive spectroscopy, transmission electron microscopy, FT-IR spectroscopy, dynamic light scattering and zeta potential. They were highly stable and, spherical in shape with the average size of 30?50 nm. The secondary metabolites involved in the formation of AgNPs were identified gas chromatography-mass spectrography. The 3D structure of the unit cell of the synthesized AgNPs was determined using XRD data base. The synthesized AgNPs exhibited significant antibacterial activity against tested bacterial pathogens, and did not show haemolysis on human red blood cells. This green synthesis could provide a new platform to explore and use AgNPs as antibacterial therapeutic agents.

Keywords： <em>Streptomyces ghanaensisem> VITHM1     nanoparticles     3D structure     antibacterial activity    

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Enhanced disinfection of <em>Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0854-x

Abstract: A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

Keywords： Photo-activated disinfection     Titanium dioxide     Nanowire membrane     Silver     Copper    

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Removable Dyes—The Missing Link for In-Depth <em>Nem>-Glycan Analysis via Multi-Method Approaches Article

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Engineering 2023, Volume 26, Issue 7,   Pages 132-150 doi: https://doi.org/10.1016/j.eng.2023.02.016

Abstract: Through the detailed structural analysis of selected, dauntingly complex <em>Nem>-glycans from chickenMoreover, this approach "visualizes" <em>Nem>-glycans that have been difficult to identify

Keywords： Glycoproteins     N-glycans     Reversible labeling     Hydrophilic interaction liquid chromatography     Capillary gel electrophoresis     Mass spectrometry    

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Novel Insight into the Etiology of Haff Disease by Mapping the <em>Nem>-Glycome with Orthogonal Mass Article

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Engineering 2023, Volume 26, Issue 7,   Pages 63-73 doi: 10.1016/j.eng.2022.09.012

Abstract: Profiling of the <em>Nem>-glycome in HD would assist in deciphering the underlying molecular mechanism<em>Nem>-glycome profiles of both serum and serum-derived immunoglobulin G (IgG) in HD were characterized

Keywords： Haff disease     Serum     Immunoglobulin G     Glycosylation     Disease pathogenesis    

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Isolation and application of predatory

Ran Yu, Shiwen Zhang, Zhoukai Chen, Chuanyang Li

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0900-3

Abstract: Indigenous predatory BALO strains were successfully isolated from activated sludge. Sludge SRF and CST were significantly reduced by BALOs induced biolysis process. The increase of BALO input dosage promoted the sludge biolysis efficiency. Sludge biolysis disintegrated flocs and lysed cells for internal water release. The optimal sludge biolysis time was 24 h and no pH adjustment was needed. -and-like organisms (BALOs) are a group of ubiquitous and obligate predatory bacteria and commonly used as biocontrol agents. In this study, an efficient, environmental-friendly, and convenient BALOs encouraged municipal waste sludge biolysis pretreatment technique was developed and investigated for dewaterability enhancement of excess waste sludge. The indigenous predatory BALOs were successfully isolated from the sludge for biolysis treatment. Without any chemical addition or pH adjustment, the sludge specific resistance (SRF) and capillary suction time (CST) were significantly reduced by as high as 53.4% and 23.8%, respectively within 24 h’s treatment, which would further be lowered with the increase of BALOs input dosage. However, the continuous extension of reaction time would worsen the sludge dewaterability. The decreases of SRF and CST accompanied with the increases of sludge disintegration degree and soluble chemical oxygen demand, nitrogen, and phosphorus concentrations all emphasized the contributions of BALOs’ predation activities to sludge disturbance, cell lysis, and consequently the release of sludge intracellular water to finally effectively improve the sludge dewaterability and disposal efficiency.

Keywords： <em>Bdellovibrioem>-and-like organisms (BALOs)     Biolysis     Activated sludge     Dewaterability     Predation    

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Title Author Date Type Operation

Individualized Pixel Synthesis and Characterization of Combinatorial Materials Chips

Xiao-Dong Xiang, Gang Wang, Xiaokun Zhang, Yong Xiang, Hong Wang

Journal Article

Direct Evidence of Coal Swelling and Shrinkage with Injecting CO2 and N2 Using <em> in-situem> Synchrotron X-ray Microtomography

Guanglei Zhang, P.G. Ranjith, Herbert E. Huppert

Journal Article

The Use of Data Mining Techniques in Rockburst Risk Assessment

Luis Ribeiro e Sousa, Tiago Miranda, Rita Leal e Sousa, Joaquim Tinoco

Journal Article

Serum <em>Nem>-Glycan Markers for Diagnosing Significant Liver Fibrosis and Cirrhosis in Chronic Hepatitis

Lin Wang, Yiqi Liu, Qixin Gu, Chi Zhang, Lei Xu, Lei Wang, Cuiying Chen, Xueen Liu, Hong Zhao, Hui Zhuang

Journal Article

Temporal and spatial stability of the EM/PM molecular subtypes in adult diffuse glioma

Journal Article

Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Journal Article

Comprehensive kinetostatic modeling and morphology characterization of cable-driven continuum robots

Journal Article

Profound Diversity of the <em>Nem>-Glycome from Microdissected Regions of Colorectal Cancer, Stroma

Di Wang, Katarina Madunić , Tao Zhang, Guinevere S.M. Lageveen-Kammeijer, Manfred Wuhrer

Journal Article

Conversion Mechanism of Biomass to Nano Zero-Valent Iron Biochar: Iron Phase Transfer and In situ Reduction

Shengnan Zhuo, Hongyu Ren, Guojun Xie, Defeng Xing, Bingfeng Liu

Journal Article

Polyolefin-nanocomposites with special properties by in-situ polymerization

Walter Kaminsky

Journal Article

VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Mani Abirami, Krishnan Kannabiran

Journal Article

Enhanced disinfection of <em>Escherichia coliem> and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Journal Article

Removable Dyes—The Missing Link for In-Depth <em>Nem>-Glycan Analysis via Multi-Method Approaches

Samanta Cajic, René Hennig, Valerian Grote, Udo Reichl, Erdmann Rapp

Journal Article

Novel Insight into the Etiology of Haff Disease by Mapping the <em>Nem>-Glycome with Orthogonal Mass

Si Liu, Yuanyuan Liu, Jiajing Lin, Bi-Feng Liu, Zhenyu He, Xiaomin Wu, Xin Liu

Journal Article

Isolation and application of predatory

Ran Yu, Shiwen Zhang, Zhoukai Chen, Chuanyang Li

Journal Article